摘要
本文采用快速脂肪提取法取代索氏提取法，提取时间仅需45min，提取率98．8％以上。并利用50m×0．35mmi．d．PEG－20M玻璃毛细管住，分析了山东黄海海域尖海龙与日本海马脂肪中的脂肪酸。其中，尖海龙分离出59个峰，鉴定了42个；日本海马分离出52个峰，鉴定了41个；分别占脂肪酸总量的97．3％和98．0％。测得尖海龙脂肪中EPA＋DHA占31．0％，日本海马为14．1％。
Fat was extracted by means of rapid fat extractor instead of Soxhlet fat extractor.With 45 min, extraction rate was over 98. 8%. Fatty acids of S. acus Linnaeus and H. japonicus Kaup near Yellow Sea of Shandong Province were separated and analyzed by a 50 m ×0. 35 mm i. d. PEG-20 M glass capillary column and the detector of FID. Of them, 59 peaks and 52 peaks were separated, 42 peaks and 41 peaks were qualified with GC/MS respectively. They were 97. 3% and 98.0% of total fatty acids. Of them, eicosapentaenoic acid and docosahexaenoic acid of S. acus Linnaeus were 31.0%, H. japonicus Kaup 14. 1%.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
1996年第2期139-143,共5页
Chinese Journal of Analytical Chemistry
关键词
气相色谱
尖海龙
海马
质谱
脂肪
脂肪酸
Capillary gas chromatography, S. acus Linnaeus, H. japonicus Kaup, gas chromatography-mass spectrometry, fat, fatty acids
