慢性苯中毒者体内部分必需元素与GST基因多态性的关系

Study on the Genetic Polymorphism of GST and Change of the Essential Elements, in Workers with Chronic Benzene Poisoning

[目的]探讨谷胱甘肽硫转移酶(GST)基因多态与慢性苯中毒患者血清中必需元素的关系。[方法]选择接触苯作业的慢性苯中毒工人为中毒组,接触苯而未发生苯中毒的工人为接触组,不接触任何工业毒物,环境及生活条件与前两组相匹配的健康人群为对照组。以含有内对照(白蛋白)的PER检测GSTT1和GSTM1的基因多态性。采用原子吸收分光光度计测定血清镁、铜、锌、铁、钙、锰、硒含量。[结果]中毒组和接触组血清镁、锰、硒均值都低于对照组,差异有显著性。中毒组中GSTM1缺失型的比例明显高于接触组和对照组(分别为52.2%、25.0%和22.5%)o中毒组GSTM1 缺失型和GSTT1缺失型的血清镁、锰、硒均值都低于对照组(P<0.01)。[结论]镁、锰、硒3种元素可能参与了毒物代谢酶GST的合成和激活过程,推测这3种必需元素的减少导致了GST基因多态性的变化;个体对苯的敏感性或耐受性存在差异的主要原因与代谢酶基因多态性有关。

[Objective] To study the relationship between essential elements and GST genetic polymorphisms in patients with chronic benzene poisoning. [ Methods ] A case-control study was conducted, 51 benzene poisoning （ BP ）workers as a case group, 61 similarly benzene-exposed workers who never suffer from BP as an exposure group and 40 normal people who have never been exposed to poisons and have similar environment and living standard with the above two groups as a control group. PCR was used to detect the GSTM1 genetic polymorphisms and GSTT1 null and non-null genotype （ albumin as an internal control ） .By using atomic absorption photospectrometer, the essential elements were determined in all groups. [ Results ] The average data of the case group and the exposure group in Mg, Mn and Se were lower than those of the control group while the difference is obvious. The percentage of GSTMI in the case group was much higher than that in the exposure group （ 52.2%, 25.0% vs 22.5% ）. The average of Mg, Mn, Se in serum of the case group was lower than that of the control group （ P 〈 0.01 ）. [ Conclusion ] The Mg, Mn, Se in chronic benzene poisoning patients and exposure patients are low. People who have GSTM1 null and low content of Mg, Mn and Se are easy to get benzene poisoning.