一种微生物检测的生物电化学方法研究

A Study on Bio-Electrochemical Method for Microbial Detection

在对细菌的代谢过程和基质氧化还原机理进行分析研究中,将生物电池应用于色素还原试验法,通过对细菌中氧化还原作用而生成的电子数的计测,以实现对细菌数的测定。由此研制成一种能快速测定奶乳制品中细菌含量的电化学生物传感器,对牛奶中的杂菌进行了测量,实验结果表明,该生物传感器能有效的测定鲜奶中的微生物含量。与检测细菌的标准平板培育法比较:该方法具有操作简单、测量速度快的特点,对同一种试样的细菌含量的测定仅需要十分钟,而用标准平板培育法至少需要二十四个小时。

The superseding process of microbes and oxidation- reduction mechanism for foundation were analyzed. Biologic cell was used to experiment of pigment reduction. Determination of germs is based on producing electron numbers in the oxidation-reduction. An electrochemical biosensor had been developed, and it was used to determine amount of germs in milk. Results show that the sensor could detect efficiently bacteria. The method, as compared with traditional method of plate cultivating germs, has more convenience on operation and fast measurement speed. For example, in the case of same sample and condition, it needs only 10 min to determine germs a time by the biosensor, but it may expend 24 h by conventional operation. In addition, the sensor can be used to detect timely microbial growing case in the solution.