金乌贼同工酶分析(英文)

Isozyme analysis of Sepia esculenta(Cephalopoda: Sepiidae)

采用水平淀粉凝胶电泳技术对金乌贼(Sepia esculenta)同工酶的组织特异性及其山东日照近海群体的遗传结构进行研究。对金乌贼眼、鳃、肌肉、口球、肝脏、鳃心6种组织的19种同工酶进行分析,检测出PGDH、GPI、MPI、IDHP、SOD、ME、AAT、DIA、MDH、LDH、G3PDH、PGM共12种同工酶在几种组织中有较稳定而清晰的表达。结果表明,金乌贼同工酶的表达有明显的组织特异性。选择金乌贼3种组织(眼、口球、鳃心)进行同工酶分析,共记录了18个基因座位,其中3个基因座位LDH-2*、G3PDH-1*、PGM*呈多态,其多态座位比例为0.166 7(P0.99)和0.055 6(P0.95),平均观测杂合度和预期杂合度分别为0.015 9和0.014 3,平均有效等位基因数为1.020 1,表明日照近海金乌贼群体的遗传多样性较低。

Horizontal starch gel electrophoresis was used to investigate the tissue-specific and genetic structure of Sepia esculenta Hoyle, 1885 （Cephalopoda： Sepiidae）. The specimens were collected from the coast of Rizhao, Shandong Province of China. A preliminary screening for 19 isozymes in six kinds of tissues （eye, branchia, mantle muscle, buccal bulb muscle, branchial heart, liver） was carried out using TC-7.0 buffer system. The results showed that the isozymes expression was highly tissue-specific in S. esculenta. Twelve isozymes （PGDH, GPI, MPI, IDHP, SOD, ME, AAT, DIA, MDH, LDH, G3PDH and PGM） and three kinds of tissues （eye, buccal bulb muscle and branchial heart） were .selected for genetic analysis of S. esculenta population. Of the eighteen putative enzyme-coding loci examined, three loci were polymorphlc, i.e., LDH-2^＊ , G3PDH-1 ^＊ and PGM ^＊ . The proportion of polymorphic loci was 0. 166 7 （P0.99） and 0.055 6 （P0.95）.The average values of observed and expected heterozygosity were 0.015 9 and 0.014 3, respectively. The average effective number of alleles was 1.020 1. Analysis of S. esculenta in the present study revealed low levels of genetic variability.