血管内皮生长因子在卵巢过度刺激综合征发病机理中的作用

Study on the role of vascular endothelial growth factor in the pathogenesis of ovarian hyperstimulation syndrome

目的探讨血管内皮生长因子(VEGF)在卵巢过度刺激综合征(OHSS)大鼠模型发病机理中的作用。方法OHSS组、控制性超排卵(COH)组和对照组每组6只未成年雌性大鼠。采用酶联免疫吸附试验方法测定大鼠血清和腹腔冲洗液VEGF水平;免疫组织化学方法和逆转录聚合酶链反应技术检测卵巢组织VEGF蛋白及其mRNA的表达。结果OHSS组、COH组和对照组大鼠的血清VEGF水平分别为(76.17±18.19)、(50.68±12.83)和(53.68±13.09)ng/L;其中,OHSS组的VEGF水平显著高于COH组和对照组(P<0.05),而COH组和对照组比较无显著性差异。OHSS组、COH组和对照组大鼠腹腔冲洗液VEGF水平分别为(17.12±1.71)(、9.38±5.88)和(6.68±1.86)ng/L;其中,OHSS组的VEGF水平显著高于COH组和对照组(P<0.05),而COH组和对照组比较无显著性差异(P>0.05)。OHSS组大鼠卵巢组织VEGF蛋白表达的平均灰度(97.23±7.26)显著高于对照组(78.55±8.48)和COH组(87.35±7.32)(P<0.05);COH组与对照组比较无显著性差异。OHSS组大鼠卵巢组织VEGF mRNA表达的灰度比(1.23±0.23)显著高于对照组(0.68±0.13)和COH组(0.92±0.07)(P<0.01),COH组也显著高于对照组(P<0.05)。结论VEGF在OHSS发病过程中发挥作用。

Objective： To explore the role of vascular endothelial growth factor （VEGF） in the pathogenesis of ovarian hyperstimulation syndrome （OHSS） in rat model. Methods： There were six rats in each of OHSS group, controlled ovarian hyperstimulation （COH） group and normal control group. The VEGF levels in serum and peritoneal washing fluid were measured by enzyme-labeled immunosorbent assay. Expressions of VEGF protein and mRNA in the ovarian tissue were detected by immunohistochemistry and reverse transcription polymerase chain reaction. Results： The serum VEGF levels in OHSS group, COH group and normal control group were （76.17 ±18. 19）ng/L, （50.68±12.83） ng/L, and （53.68±13.09） ng/L, respectively, and the serum VEGF levels in OHSS group were significantly higher than those in COH group and normal control group （P〈0.05, P〈0.05 respectively）. The peritoneal VEGF level in OHSS group was 17.12 ±1.71 ng/L, which was significantly higher than that in COH group （9.38±5.88） ng/L and normal control group （6.68±1.86） ng/L （P〈0.05, P〈0.05 respectively）. No significant difference was found between COH group and normal control group. The expression of VEGF protein in ovarian tissue of OHSS group was higher than that of normal control group （P〈0.05） and COH group （P〈0.05）. But there was no significant difference in expression of VEGF protein between COH group and normal control group. The expression of VEGF mRNA in ovarian tissue of OHSS group was higher than that of normal control group and COH group （P〈0.05, P〈0.05 respectively）. Conclusions. VEGF may play a role in the pathogenesis of OHSS.