LED照射对溃疡性结肠炎大鼠结肠粘膜组织的修复作用

The Effects of LED Rectal Irradiation on the Experimental Ulcerative Colitis in Rats

研究发光二极管(light-emittingdiode,LED)直肠内照射对大鼠实验性溃疡性结肠炎(ulcerativecolitis,UC)的抗过氧化损伤以及促进结肠粘膜组织的修复作用。34只大鼠随机分成3组:正常对照组10只、病理模型组12只与病理模型+LED照射治疗组12只.研究中采用乙酸局部刺激复制大鼠UC模型,其中LED治疗组应用LED结肠内照射,1日1次,共10d。其后观察各组的病理变化,血清过氧化损伤水平。结果显示,LED结肠内照射能够促使实验性溃疡性结肠炎的组织修复;显著降低溃疡性结肠炎大鼠血清MDA水平、升高SOD活性。实验表明LED结肠内照射实验性溃疡性结肠炎大鼠具有抗过氧化损伤作用以及促进结肠粘膜组织的修复作用。

In this paper, the effect of light emitting diode （LED）（LED λ 632.8 nm; power 4.0 mW）irradiation directly to the colon on the experimental ulcerative colitis （UC） were studied. 34 rots were made into 3 groups： LED curative group （n= 12）, model group （n= 12） and normal control group （n = 10）. Glacial acetic acid （5%） was used intra-anally to replicate the rat model of ulcerative colitis. After a week treatment with administrating LED rectal irradiation to curative group, 30 rain each time, once each day, the histopathological studies in Colonic tissue were performed, and the activity of supemxide dismutase （SOD） and content of malondi-aldehyde （MDA） in the blood plasma were detected. The extent of the colonic tissue injury in curative group was not as significant as that in the model group. Comparing with model group, the content of MDA in LED curative group was reductive and the activity of SOD was increased significantly. We found that the LED irradiation can protect colonic mucosa from acetic acid induced tissue damage and oxidative stress in rots and the effects may be related to the photobiomodulation of LED.