大鼠高尿酸血症肾损害模型的建立

Establishment of Hyperuricemic Renal Impairment Model in Rats

目的建立大鼠高尿酸血症肾损害动物模型。方法采用腺嘌呤加乙胺丁醇给高、中、低3个剂量组灌胃,测定实验前后大鼠血清尿酸、尿素氮、肌酐,行肾组织病理与超微病理观察。结果高剂量组、中剂量组血尿酸升高,高剂量组肾损害严重。结论中剂量组作为高尿酸血症动物肾损害模型较为合适。

Objective To establish a hyperuricemic renal impairment model in rats. Methods High, moderate and low dose of adenine and ethambutol were used to Wistar rats. Concentration of serum uric acid and urine nitrogen and creatinine was measured before and after treatment. The histopathological and uhrastructural changes were examined by light and electron microscopy. Results Serum uric acid was increased in the high and moderate dose groups, and the high dose group showed the most severe lesions. Conclusion The moderate dose treatment is suitable for preparation of hyperuricemic renal impairment model in rats.