用高效液相色谱法测定氨咖黄敏胶囊三组分的含量

The Determination of Three Components in Ankahuangmin Capsule by HPLC

目的:采用高效液相色谱法(HPLC)同时测定氨咖黄敏胶囊中对乙酰氨基酚、咖啡因、马来酸氯苯那敏的含量.方法:采用Waters Nova-pakC18(150mm×3.9mm,4μm)色谱柱,乙腈-0.03mol/L磷酸氢二铵溶液为流动相,流速为1.0ml/min,检测波长214nm,柱温30℃.结果:采用HPLC测定对乙酰氨基酚、咖啡因和马来酸氯苯那敏的含量,线性范围分别为205.6～1233.8μg,12.16～72.96μg,1.03～6.18μg,r=0.9997,0.9999,0.9999;平均回收率分别为99.5%(RSD＝0.3%),99.5%(RSD＝0.5%),99.8%(RSD＝0.8%).结论:HPLC法简便、快速、准确,用此法同时测定氨咖黄敏胶囊中3个组成成分的含量,能更好地控制产品质量.

Objective： To determinate the contents of acetaminophen, caffeine, and chlorphenamine maleates in the Ankahuangmin Capsule with HPLC. Methods： The column used in the analysis was Waters Nova-pak C18 （150 mm × 3.9 mm,4 μm） at 30 ℃, with the mobile phase of acetonitrile-0. 03 moL/L diammonium hydrogen phosphate （adjusted with phosphate acid to pH3. 1 ） （ 12：88 ） containing 0. 75 mmoL/L heptane sulfonic acid sodium. The flow rate of the mobile phase was 1.0 ml/min. The wavelength of the UV detector was 214 nm. Result： The linear range of acetaminophen, caffeine, chlorphenamine maleates were 205. 6 ～ 1 233.8, 12. 16 ～72. 96, 1.03 ～6. 18 lμg/ml,and the coefficiency were r = 0. 999 7, 0. 999 9,0. 999 9 respectively. The recovery rates were 99. 5% （ RSD =0. 3% ）, 99. 5% （ RSD =0. 5% ）, and 99. 8% （RSD =0. 8% ）respectively. Conclusions： The HPLC method is simple, easy to operate, and can be used in quality control of Ankahuangmin Capsule.