谷胱甘肽硫转移酶M1基因多态与肺癌易感性的关系

Glutathione S-transferase M1 Polymorphisms and Susceptibility to Lung cancer

目的探讨谷胱甘肽硫转移酶M1(GSTM1)基因多态与支气管肺癌癌变的关系。方法采用回顾性“病例—对照”设计和限制性片段多态检测法(PCR-RFLP),检测肺癌病例组103例和正常对照组138例的GSTM1基因多态,以非条件性logistic回归模型分别对年龄、性别进行校正后计算比数比(OR)及95%可信区间(CI)。结果GSTM1的缺陷型基因(D)频率在对照组、肺癌组分别为44.2%和61.2%。logistic回归分析表明,GSTM1缺陷型(D)患肺癌的危险度升高2.09倍,差异有统计学意义。GSTM1(D)可显著增加鳞癌和小细胞癌的患病危险度。结论GSTM1(D)是患肺癌的危险因素,GSTM1(D)存在与吸烟有协同作用。

Objective To study the relationship of glutathione S - transferase M1 （ GSTM1 ） polymorphisms with occurrence of bronchic lung cancer. Methods The case - control study and PCR - RFLP was conducted among 103 subjects and 138 healthy controls, and the genetic polymorphism of GSTM1 were detected. Logistic regression analysis were used to assess the value of odds ratios （OR） and 95% confidential intervals （CI） between different genotypes and smoking after adjusted by ages and gender. Results For GSTM1 , the allele frequencies of deletion（D） were 44.2% and 61.2% in control and LC groups, respectively. The adjusted OR of the GSTM1 deletion （D） genotype showed a 2.09 fold increased risk for LC. The GSTM1 deletion genotypes（D） increased risk for developing SCC and SC, significantly. Conclusion The mutant genotypes GSTM1 deletion polymorphisms raised risk of LC in the population. It is reasonable that GSTM1 deletion genotypes（D） increased risk for developing SCC and SC, obviously. The two genetic polymorphisms and smoking have the joint effect.