摘要
①目的探讨在直线加速器照射下,低浓度氧吸入、碳合氧吸入以及高-低氧吸入等三种不同的方式对小鼠骨髓细胞放射生物效应的影响。②方法昆明小鼠随机分为空气组,高氧组,低氧组,高-低氧组。用6MV-X线不同剂量全身单次照射,配合剂量效应曲线,求出不同吸氧方式的放射防护系数(RPF)并用图像分析仪测定白细胞介素-3(IL-3)基因蛋白相对含量。③结果高氧组碳合氧的吸入显示了放射增敏作用,其RPF为0.86,低氧组低氧放疗具有放射防护作用,其RPF为1.41,高-低氧组高-低氧放疗也表现出一定的放射防护作用,RPF为1.09;高-低氧组与空气组IL-3基因蛋白的表达差异无统计学意义(F=5.37,q=2.87,P>0.05),而高氧组和低氧组与空气组相比,IL-3基因蛋白的表达分别减少和增加,差异有统计学意义(q=3.37、4.23,P<0.05)。④结论IL-3的免疫组化定量表达可能是辐射所致骨髓早期损伤的分子标志。高-低氧放疗可消除高氧加重的骨髓辐射损伤,在一定程度上具有放射防护作用。
Objective To evaluate the effects of several ways of oxygen inhalation on radiation response of murine bone marrow. Methods Kunming mice were divided into four groups;Air,HO,LO and HO＋LO. The mice were subjected to whole- body irradiation with 6 MV X-rays in single dose. The relationship between the micronucleus frequency of polychromatic erythrocytes （PCES） and radiation dose was established, dose-effect curves were fitted. Radioprotective factor （RPF） was calculated. Results RPF was 0.86, 1.41, and 1.09 in the HO, LO and HO＋LO group, respectively. The difference of expression of IL-3 between HO＋LO group and Air group was not significant （F=5.37,q=2.87 ,P〉0.05）, while that difference of IL-3 in HO group and LO group was significant compared with the Air group （q=3. 37,4. 23 ;P〈0.05）. Conclusion The expression of IL- 3 may be an index of radiation damage on the bone marrow in earlier stage. Carbogen plus low- concentration oxygen radiotherapy eradicate the enhanced damage of irradiation on bone marrow by carbogen, and indicate of radio-protective effects some degree.
出处
《齐鲁医学杂志》
2005年第4期315-317,320,共4页
Medical Journal of Qilu
