猪应激敏感性生化遗传标记的研究

A STUDY ON BIOCHEMICAL GENETIC MARK OF THE PORCINE STRESS SUSCEPTIBILITY

８３头猪用于ＰＨＩ、ＰＧＤ、ＰＯ＿２生化多态性分析，结果显示：在３种表型分布内，氟烷阳性猪发生率、背最长肌ｐＨ值、系水力等肉质性状差异极显著（Ｐ＜０．０１）；同一个优良基因数目与氟烷敏感性、肉质指标呈强线性关系。对四种亲本交配组合共计１９窝１３２头猪的亲子代进行了ＨＭ单倍型推断的遗传分析，结果表明：前三种交配组合可以根据亲子代的ＰＨＩ、ＰＯ＿２：、ＰＧＤ表型及子代的氟烷测验结果对ＨＭ单倍型进行推断；Ｈａｌ￣ｎ与ＢＳＡ、ＢＳＢ连锁紧密，Ｈａｌ￣Ｎ与ＡＳＡ、ＡＦＡ连锁紧密。用已知双亲的ＨＭ单倍型，结合子代的酶型推断氟烷基因型，准确率达９０％。因此，ＰＨＩ、ＰＯ＿２、ＰＧＤ表型可以作为猪应激敏感性的生化遗传标记。

Eighty-three pigs were used to study the biochemical polymorphisms of PHI,PO_2and PGD,The results showed that there tvere significantly different(P<0.01)in thedistribution of halothane-positive pigs,muscular pH and water holding capacity amongthree phenotypes of PO_2,PHI and PGD respectively.The amounts of PHI￣A,and PGD￣B allels in a pig were linearly related to halothane sensitivity and meat qualitytraits respectively.Four types of parental mating combination from 132 piglets in 19 litters were included in the experiment.The first 3 types of parental mating combina-tion could be used to deduce the HM haplotype of parents and offsprings.The resultsshowed that Hal￣n was closely linked with BSA and BSB while Hal￣N was closely linkedwith ASA and AFA. Using the haplotype(deduced)of parents in combination with thephenotypes of PHI,PO_2 and PGD of piglets,piglets halothane genotype were deducedand the deduced accuracy came to 90 per cent.Therefore,it can be said that thephenotypes of PHI,PO_2 and PGD can be used as biochemical genetic mark of porcinestress susceptibility.