乳牛肝功能与维生素D_3代谢及骨软病关系的研究

STUDY ON RELATIONSHIP OF LIVER FUNCTION AND VITAMIND3 METABOLISM AND OSTEOMALACIA IN DAIRY COWS

通过血常规、血清钙、无机磷、碱性磷酸酶活性及其同工酶酶谱、尾椎Ｘ线检查等方法，在北京市郊区某农场，选出１０头健康成年黑白花乳牛作为对照组，１０头骨软病乳牛作为试验组。分别对其肝功能、血清维生素Ｄ＿３，２５－羟维生素Ｄ＿３（２５－ＯＨ－Ｄ＿３）和１，２５－双羟维生素Ｄ＿３［１，２５－（ＯＨ）＿２－Ｄ＿３］进行测定。结果表明：骨软病乳牛血清硫酸锌浊度及γ－球蛋白明显高于健康水平（Ｐ＜０．０５和Ｐ＜０．０１），而血清白蛋白则明显低于对照组（Ｐ＜０．０１）。骨软病乳牛血清２５－ＯＨ－Ｄ＿３显著低于健康牛（Ｐ＜０．０１），维生素Ｄ＿３和１，２５－（ＯＨ）＿２－Ｄ＿３极显著低于健康乳牛（Ｐ＜０．０１）。据此可以确认，乳牛肝功能不全可影响维生素Ｄ＿３的吸收和羟化，进一步导致骨软病的发生。

Ten healthy cows were selected out as the control group and ten cows with osteo-malacia were selected out for the experimental group on Beijing one suburban farmaccording to routine blood test, the concentrations of serum calciuzn, inorganic phosph-orus, activityof alkaline Phosphatase (AKP) and AKP isoenzymes zymogram, andfindings of tail vertera x-ray examination. Parameters of liver function and the concen-trations of serum vitamin D_3, 25-OH-D_3, 1, 25-(OH)_2-D_3 were measured. Theresults shows: Zn T. T. and the concentrations of serum Y-globulin are inarkly higher(P<0.05) in the sick cows than that in health cows, the concentrations of serumalbumin are briefly lower (P<0.05) in the sick cows than those in health cows. Theconcentrations of serum vitamin D_3, 25-OH-D_3 ,1, 25-(OH)_2-D_3 are markly lower(P<0.05 or 0.01) in the cows with osteomalacia than those in the health cows. Theresults indicated that first liver dysfunction could affected on absorption and hydroxy-lation of vitamin D_3 and further caused osteomalacia.