川芎嗪对大鼠急性坏死性胰腺炎微循环与炎症介质的影响

Protective Effect of Ligustrazine on Intestinal Microcirculation and Inflammatory Mediates of Acute Necrotizing Pancreatitis in Rats

目的:探讨急性坏死性胰腺炎(acute necrotizing pancreatitis,ANP)时肠组织血流量及炎症介质变化对肠损伤的影响及川芎嗪的保护作用。方法:SD大鼠96只,随机分为对照组(C组)、胰腺炎组(P组)和治疗组(T组)。以5%牛磺胆酸钠胰腺被膜下均匀注射复制ANP模型。采用放射性生物微球技术在制模后2 h及12 h分别测定肠组织血流量,同时检测血清磷脂酶A2(PLA2)活性及白细胞介素-1β(IL-1β)水平,并观察肠黏膜病理改变。结果:P组在制模后2h及12 h肠组织血流量(0.80±0.07,0.50±0.06)m l.(m in.g)-1较C组(1.56±0.18,1.61±0.11)m l.(m in.g)-1明显减少(P<0.001),血清PLA2活性(94.29±9.96,103.71±14.40)U/L,IL-1β水平(0.78±0.13,0.83±0.20)μg/L较C组(65.27±10.52,66.63±9.81)U/L,(0.32±0.06,0.33±0.07)μg/L明显升高(P<0.001),肠黏膜损伤程度较C组明显加重(P<0.001)。与P组比较,T组在制模后2h及12h,肠组织血流量(1.05±0.12,0.92±0.08)m l.(m in.g)-1较P组明显增高(P<0.01),血清PLA2活性(81.77±8.95,84.02±9.01)U/L,IL-1β水平(0.54±0.07,0.48±0.05)较P组明显降低(P<0.05),肠黏膜组织损伤程度较P组明显减轻(P<0.05)。结论:ANP早期肠组织血流量减少与炎症介质的升高同时发生,两者均是ANP时肠黏膜损伤的重要因素,川芎嗪对ANP肠损伤具有保护作用。

Objective ： To explore the protective effect of ligustrazine on the changes of intestine injury, intestinal microcireulation and inflammatory mediators in acute necrotizing pancreatitis （ANP）. Methods： A total of 96 rats were randomized into control group （ C ）, pancreatitis group （P） and treatment group （T）. The model of ANP established by injection of 5% sodium taurocholate into the pancreatic capsule. Radioactive biomicrosphere technique was applied to measure the intestinal blood flow at 2 h, 12 h after ANP. Simultaneously, blood samples and intestine tissues were collceted to observe the changes of phospolipase A2 （ PLA2 ）, Interleukin-1β （ IL-1β ） and histological exam. Results ： Comparing with C group, the intestinal pathological changes in P group were significantly aggravated （ P 〈 0.001 ）, intestinal blood flow was significantly decreased （ P 〈 0. 001 ）, serum PLA2 activity and IL-1β level were elevated significantly （P 〈0. 001 ） in all phases. Contrast with P group, the intestinal pathological changes in T group were significantly alleviated （P 〈0.05 ）, the intestinal blood flow was increased significantly （P 〈 0.01 ）, serum PLA2 activity and IL-1β level were decreased significantly （ P 〈 0.05 ） in all phases. Conclusion ： The decrease of intestinal blood flow and increase of inflammatory mediates happened at the same time in the early stage of ANP. Implicating these both changes were the essential factors of intestinal injuries. Our experiments demonstrated that ligustrazine had protective effect on intestinal injuries.