中国镇江地区幽门螺杆菌vacA基因型状况及其与胃十二指肠疾病关系的研究

The Investigation of vacA Genotype of Helicobacter Pylori and the Relationship Between the Presence of Specific Genotypes and Gastroduodenal Diseases in Zhenjiang of China

目的:调查中国镇江地区幽门螺杆菌(H.pylori)vacA基因型状况及其与胃十二指肠疾病关系。方法:在微氧环境下取胃黏膜标本中分离培养出H.pylori共36株,以聚合酶链反应(PCR)方法检测其基因组DNA中vacA基因型。结果:36株H.pylori临床株中vacA信号序列类型中s1 a、s2所占比例分别为83.3%、13.9%,未发现s1b型。vacA中间序列类型中m1、m2所占比例分别为16.7%、75%。分别有1株和3株vacA信号序列和中间序列未能分型。基因型组合s1 a/m2、s1 a/m1、s2/m2所占比例分别为61.1%、19.4%、8.3%。H.pylori菌株vacA各基因型在胃炎、胃溃疡、十二指肠溃疡和胃癌四组疾病中的分布差异均无显著性(P>0.05)。结论:s1 a、m2和s1 a/m2分别是中国镇江地区H.pylori菌株vacAs、m区及其组合的优势基因型。但本研究未能证实H.pylori菌株vacA基因型与胃十二指肠疾病的相关性。

Objective： To investigate the status of Helicobacter pylori （H. pylori） vacA gentype and the relationship between the presence of specific genotypes and gastroduodenal diseases in Zhenjiang of China. Methods： All of 36 H. pylori strains was cultured from gastric biopsies under microaerobic conditions and determined the vacA genotypes of its genomic DNA with polymerase chain reaction. Results： In the genttype of vacA signal sequences of 36 H. pylori strains, the proportions of sla and s2 was 83.3% and 13.9% while sl b was not found. In the genotype of vacA midregion sequences, the proportions of ml and m2 was 16.7% and 75%. There were one and three H. pylori strains which could not identified the genotype of signal and midregion sequences. The proportions of sla/m2, sl a/ml, s2/m2 in genotype compose were 61.1%, 19.4%, 8.3%, respectively. There were no significant differences between the vacA genotype with the four gastroduodenal diseases（ P 〉 0.05 ） Conclusion ： sla, m2 and sla/m2 were the dominant s, m genotype and genotype compose of H. pylori in Zhenjiang of China. However, the relationship between vacA genotypes of H. pylori and the gastroduodenal disease can be identified in this study.