大豆抗锈病组织病理学研究

STUDY ON THE HISTOPATHOLOGY OF SOYBEAN RESISTANCE TO RUST

以抗感品种ＰＩ４５９０２５（Ｒ）和猴子毛（Ｓ）为试材．人工接种大豆锈菌（ＰｈａｋｏｐｏｒａｐａｃｈｙｒｈｉｚｉＳＹｄ．）后第１天起每２天取样１次（共７次），石蜡切片及电镜扫描。大豆锈菌在抗感品种上均是从表皮角质层直接侵入，侵入时均产生木质化，但抗病品种木质化速度快且数量大。抗病品种锈菌侵入后能产生乳状突起结构，且完成一次侵染至少需１４－１５天，而感病品种猴子毛则只需８－９天。接种后１３天产生的夏孢子堆感病品种是抗病品种的２１．３倍。抗感品种间表皮叶毛形态及排列亦存在差异．

A study of the histopathology on two typical resistant and susceptible soybean varieties PI459025(R)and Houzimao (S)was conducted and the results showed that thepenetration path of soybean rust on the two varieties were directly through culticle from their leaf surface, the penetration speed of soybean rust was faster in susceptible variety than in resistant one,and quantity of uredinia formed in susceptible variety was more than in resistant variety. The speed of lignification in resistant variety was faster and the quantity oflignification was more than in susceptible variety .and papillae-like structure was observed in resistant variety. The observation of structure of leaf hairs under SEM showed that the leaf hairs of PI459025(R)were bulky,short and regular line,while the leaf hairs of Houzimao (S)were slender.long and irregular line.