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pcDNA3.1F9质粒介导人凝血因子IX在肠上皮细胞中的表达 被引量：1

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摘要 目的探索肠上皮细胞用于血友病B基因治疗的可能性。方法用已构建好的质粒载体pcDNA3.1F9,以脂质体介导法转染肠上皮细胞SW480,RT-PCR检测mRNA的转录,ELISA、一期法检测蛋白表达及凝血活性。结果转染后的细胞中有人凝血因子IX(humancoagulationfactorIX,hFIX)mRNA的转录;ELISA法测得转染后24h细胞上清中的hFIX蛋白量为(11.72±0.24)ng·106cell-1·24h-1,转染后48hhFIX蛋白量为(381.34±16.16)ng·106cell-1·24h-1。一期法结果示细胞上清中的hFIX有凝血活性,转染后48h达(45±0.85)%·106cell-1。结论肠上皮细胞SW480转染pcDNA3.1F9质粒后可表达有凝血活性的hFIX,肠上皮细胞有望成为血友病B基因治疗的靶细胞。
作者 杜建伟 黄细莲 文路 陈方平 傅敢 付斌
机构地区 中南大学湘雅医院血液科
出处 《中国医师杂志》 CAS 2005年第9期1207-1208,共2页 Journal of Chinese Physician
关键词 肠上皮细胞 凝血因子IX 基因治疗 人凝血因子IX 质粒介导 RT-PCR检测 细胞SW480 ELISA法 hFIX 脂质体介导法
分类号 R554 [医药卫生—血液循环系统疾病]
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参考文献5

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同被引文献28

  • 1李向臣,任芳丽,张涌.山羊乳腺上皮细胞的分离、培养与超微结构观察[J].畜牧兽医学报,2005,36(2):121-126. 被引量：27
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中国医师杂志
2005年 第9期

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