肝硬化和肝癌组织中乙型肝炎病毒、丙型肝炎病毒及p53蛋白的检测

Measurement hepatitis B,C virus and p53 protein in cirrhosis and hepatocellular carcinoma

目的检测肝硬化和肝细胞癌(hepatocellular carcinoma,HCC)组织中乙型肝炎(hepatitis B virus,HBV)表面抗原(HBsAg)、丙型肝炎(hepatitis C virus,HCV)核心抗原(HCVAg)和p53蛋白的表达。方法应用免疫组织化学法检测20例肝硬化,78例HCC及癌旁肝组织HBsAg、HCVAg和p53蛋白的表达。结果肝硬化,HCC及癌旁肝组织中HBsAg、HCVAg、HBsAg并HCVAg和p53蛋白表达率分别为75.0%、40.0%、25.0%2、0.0%;9.07%、6.4%、2.6%、87.2%;80.8%、44.9%、29.5%、21.8%,3组间有显著性差异(P<0.01)。HCC与癌旁肝组织之间HBsAg、HCVAg、HBsAg并HCVAg和p53蛋白阳性表达率有显著性差异(P<0.01)。在肝硬化和HCC无肝硬化组,p53蛋白表达与HBsAg阳性率显著相关(P<0.01)。HCC合并肝硬化组p53蛋白表达与HBsAg、HCVAg、HBsAg并HCVAg阳性率显著相关(P<0.01)。结论HCC的发生与HBV和HCV的感染密切相关,肝细胞癌变是一个长期的、多因素作用的结果,在这个过程中p53基因的突变起到了重要的作用。

Objective To detect Hitching, HCVAg and p53 protein in cirrhosis and HCC. Methods Immunohistoclacmical method was used to detect HBsAg, HCVAg and p53 protein in 20 cirrbosis, 78 HCC and paracarcincma hepatic tissues. Results The positive expressions of HBsAg, HCAVg,HBsAg with HCVAg, and p53 protein in cirrhosis, HCC and paracarcinoma hepatic tissues were 75.0%, 40.0%, 25.0%, 20.0% ; 9.0%, 6.4%, 2.6%, 87.2% ; 80.8%, 44.9%, 29.5%, 21.8% respectively, with significant difference （P〈0.01） among three groups.There has significant difference（P〈0.01） between HCC and paracarcincma hepatic tissue.The positive expression of p53 protein was closely related to that of HBsAg in cirrhosis and HCC without cirrhosis （ P〈0.01）. The positive expression of p53 protein was strongly liked to that of HBsAg, HCVAg, HBsAg with HCVAg in HCC with cirrhosis （ P〈0.01）. Conclusion HBV and HCV are strongly liked to HCC. Hepatocarcinogenesis is a long process relating to many factors. This result shows that mutation of p53 gene plays a important role in this process.