犊牛腹泻轮状病毒在3个传代细胞上培养特性的研究

A Study of the Biological Charactor of Bovine-RV Culture in Three Cell Iines

对犊牛腹泻轮状病毒（ＮＣＤＶ）在３种传代细胞系上进行了培养，并将其培养特性进行了比较研究。结果表明：犊牛腹泻轮状病毒在ＭＡ－１０４细胞上生长，其病毒抗原的生长部位在细胞浆，培养后可出现细胞病变；犊牛腹泻轮状病毒在ＬＬｃ－ｍｋ２细胞上也生长，且随着培养代次的增加，病毒抗原的滴度相应增加，增长到一定滴定后即较稳定。

In this study, the Bovine-Rv has been cultured with three cell lines (MA-104 lineand LLc-Mk2line and Hela line) and the biological charactors have been compared amongthese three cell lines: The result is that Bovine RV CAN GROW ON MA-104 with its growthposition being in its cytoplasm; After infected with Bovine Rv, obvious cytopathic effects(cpe) appear on MA-104 Cells. Bovine Rv can grow on LLc-Mk2Cells and the growth position of Bovine Rv of LLc-Mk2Cells is also in the cytoplasm,The more generation is Cultured,The more antigent cells oF Bovine Rv can be detected by IFA and EIA; when the Bovine Rvhas increased to a certain degree in the LLc-Mk2cells, The number of cells of Bovine Rv become stable, But cytopathic effects (cpe)cannot appear on the LLc-Mk2Cells infected withBovine Rv; Bovine Rv cannot grow in Hela cells.