小鼠2细胞胚卵裂球电融合的研究

Electrofusion of Blastomeres From Mouse 2-And 4-Cell Embryos

分别用８００ｖ／ｃｍ、４０～８０μｓ的单个电脉冲；８００ｖ／ｃｍ、１６０～３２０μｓ的单个电脉冲；１０００ｖ／ｃｍ、１６０～３２０μｓ的单个电脉冲；１２００ｖ／ｃｍ、１６０～３２０μｓ的单个电脉冲；１０００ｖ／ｃｍ、１６０～３２０μｓ的２个电脉冲所获得的胚胎融合率分别为８４．６％（３３／３９），１００．０％（５９／５９），９７．４％（７４／７６），９５．１％（３９／４１），６４．７％（１１／１７）。融合胚作培养后，２细胞胚的发育率分别为１５．２％（５／３３），３９．０％（２３／５９），４７．３％（３５／７４），４３．６％（１７／３９），１８．２％（２／１１）。以１０００ｖ／ｃｍ，１６０～３２０μｓ的单个脉冲诱导注射ＬＨ３９～４３ｈ，４４～４８ｈ回收的２细胞胚，胚胎融合率分别为１００．０％（２７／２７），９６．４％（２７／２８）；发育率分别为４４．４％（１２／２７），３３．３％（９／２７）。２细胞胚卵裂球融合的适宜条件为１０００ｖ／ｃｍ和１６０～３２０μｓ的单个脉冲。

The fusion rates of .mouse blastomeres from 2-cell embryo were 84.6%(33/39),100.0% (59/59),97.4% (74/76), 95. 1% (39/41), 64.7% (11/17) respectively, When embryos were treated at such electrofusion parameters that is one 800v/cm,from 40 to 80μsDC pulse,two1000v/cm,from 160 to 320us DC pulse and one 160-320μs pulse of 800,1000, 1200v/cm,and its development rates of 2-cell embryo were 15. 2 % (5/33),39.0%(23/59),47.3%(35/74),43.6% (17/39),18.2% (2/11) respectively. When the embryos were recovered at the time of 39～43 hours or 44～ 48 hours after LH injection and were treated with one 1000v/cm, DC pulse of 160～320μs, the fusion rates were 100.0% (27/27),96.4% (27/28),and its developmental rates were 44.4%(12/27),33.3% (9/27) respectively.The effectual electrofusion parameters were one 1000v/cm from 160 to 320μs DC pulse.