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磁性固定化漆酶在苹果汁除酚中的应用 被引量：3

The Characterstics of Magnetic Immobilized Laccase and Its Application on Production of Apple Juice
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摘要 以磁性聚苯乙烯微球为载体,戊二醛为交联剂,制备出了磁性固定化漆酶。研究表明,固定化漆酶的Km值较游离漆酶小,最适温度与游离漆酶相同,最适pH较游离漆酶下降0.4个单位,热稳定性、pH稳定性和贮存稳定性均较游离漆酶有所提高。 A magnetie immobilized laecase has been prepared by immolilizing laecase onto magnetic polystyrene microspheres with glutanaldehyde.The measured Km values in magnetic immobilized enzyme were lowwer than free enzyme.The optimum lemperature of magnetie immobilized enzyme was same as free enzyme.The optimum pll of magnetic immobilized enzyme was 0.4 unit lower than that of free enzyme.Comparaed with free enzyme magnetic immobilized enzyme has more excellent thermal stability.pH stability and storage stability.
作者 张寒飞 杨婉身 黄乾明
机构地区 四川农业大学生命科学与理学院
出处 《保鲜与加工》 CAS 2005年第5期15-18,共4页 Storage and Process
关键词 磁性固定化漆酶 苹果汁 多酚 magnetic immobilized lacease apple juice polyphenol
分类号 TS255.44 [轻工技术与工程—农产品加工及贮藏工程]
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参考文献10

  • 1Thurston C F. The structure and function of fungal laccase [J]. Microbiology, 1994,140: 9-26.
  • 2Bourbonnais R, Paice MG,Freiemuth B, et al.Reactivity of various mediator and laccase with kraft pulp and lignin model [J ].Environ Microbio l,1997, 63 ( 12 ): 4627-4632.
  • 3Collins P J, Koterman M J J, Field J A,et al. Oxidation of anthracene and benzo[a]pyrene by laccase from trametes versicolor [J]. Applied and Environmental Microbiology, 1996,62: 4563-4567.
  • 4Szeponic J, Mǒller B,Pfeiffer D, et al. Ultrasensitive bioenzyme sensor for adrenaline[J].Biosensor Bioelectronics, 1997,12 : 947-952.
  • 5葛毅强,蔡同一,胡小松.果汁二次混浊研究的新进展[J].食品与发酵工业,2002,28(9):46-50. 被引量：33
  • 6D'Annibale A, Stazi S R, Vinciguerra V, et al. Oxirane-immobilized Lintinula edodes laccase: stability and phenolics removal efficiency in olive mill wastewater [J ]. J Biotech, 2000, 77:265-273.
  • 7Palmieri G, Giardina P,Bianco C, et al. A novel white laccase from Pleurotus ostreatus [ J ].J Biol Chem, 1997,272: 31301-31307.
  • 8Rogalski J, Dawidowicz A, Józwik E, et al. Immobilization of laccase from cerrena unicolor on controlled porosity glass [J].J Molecular Catalysis B: Enzymatic, 1999(6): 29-39.
  • 9Bradford M M.A rapid and sensitive method for the quantitation of microgram quantities of protein using the ptinciple of protein-dye binding [ J ]. Anal Biochem, 1970,72: 248-254.
  • 10刘淑珍,钱世钧.担子菌漆酶的分离纯化及其性质研究[J].微生物学报,2003,43(1):73-78. 被引量：51

二级参考文献18

  • 1Yoshida H.Chemistry of Lacquer(Urusht)part 1.J Chem Soc,1883,43:472～486.
  • 2Bertrand G.Sur la presence simultanee de la laccase et de la tyrosinase dans le suc de quelques champignons.C.R.Hebd Seances.Acad Sci,1987,123:463～465.
  • 3Laborde J.Sur la casse des vins.C.R.Hebd Seances.Acad Sci,1896,123:1074-1075.
  • 4Givaudan A,Effosse A,Faure D,et al.Polyphenol oxidase from Azospirillum lipofeum isolated from the rhizosphere:evidence for a laccases in non-motile strains of Azospirillum lipoferum.FEMS Microbiol lett,1993,108:205～210.
  • 5R.Loitie. Laccase in copper proteins and copper enzymes,Vol 3. Boca Raton Fla: CRC Press Inc.1984.1～35.
  • 6Robert Bourbonnais,Michael G Paice.Demethylation and delignification of kraft pulp by Trametes versicolor in the presence of 2,2'-azinobis-(3-ethylbenzthi-azoline-6-sulphonate).Applied Microbiology and biotechnology,1992,36(6):823～827.
  • 7O H Lowry,et al.Protein measurement with the Folin phenol reagent.J Biol Chem,1951,193:265～275.
  • 8钱世钧 郝凤兮 孟广震.大肠杆菌AS1.375 L—天门冬酰胺酶的选择性化学修饰[J].微生物学报,1984,24(4):352-356.
  • 9Kurtz M B,Champe S P.Purification and characterization of the conidial Laccase of Aspergillus nidulans.J Bacteriol,1982,15:1138～1345.
  • 10Cheung D S M,Marshall K C.Antigenic and some kinetic properties of three p-diphenol oxidase isoenzymes of Trametes versicolor. Biochim Biophys Acta,1969,178:177～180.

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保鲜与加工
2005年 第5期

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