山麦冬的HPLC/ESI-MS特征指纹图谱研究

HPLC/ESI-MS fingerprint of Radix Liriopes

目的研究山麦冬Liriopespicata甲醇提取物中的物质组成情况,建立山麦冬HPLC/ESI-MS特征指纹图谱。方法山麦冬用甲醇超声提取,提取液经C18SPE固相萃取柱除去多糖成分后,用高效液相色谱-电喷雾离子阱质谱(HPLC/ESI-MS)分析提取物中的物质组成。结果山麦冬甲醇提取物色谱图中有17个共有峰的重现性与精密度的RSD<5%,建议以此为基础制作山麦冬特征指纹图谱。结论应用HPLC/ESI-MS技术建立的山麦冬指纹图谱特征性强、重现性较好,可用于山麦冬的品质评价与品种鉴别。

Objective To study the compositions of methanol extracts from Radix Liriopes （RL） and establish the characteristic fingerprint of RL by HPLC/ESI-MS. Methods First, the samples of RL were extracted by ultra-sonication using methanol as extracting solvent. Then the methanol extracts of RL were pretreated by AICHROMTM Reliz Sil C18 column to remove polysaccharides. Finally, the cleaned samples were injected into a high performance liquid chromatography coupled with electrospray-ionization ion trap mass spectrometry electrospray to analyse their composistions. Results In the chromatogram of the methanol extracts from RL, there were 17 common peaks with the RSD of reproducibility and precision less than 5%. Conclusion The characteristic fingerprint of the RL established by HPLC/ESI-MS technology is characteristic and reproducible, which could be applied to the quality assessment and variety identification of RL Spicate.