肺炎克雷伯菌体外耐药监测

Distribution and drug resistance of clinical isolatesof Kiebsiella pneumoniae

目的探讨肺炎克雷伯菌(KP)的分布、感染特点及其体外对抗生素的耐药情况.方法采用纸片扩散法K-B法对武汉大学人民医院临床分离的130株KP进行耐药性检测,并用双纸片协同法初筛、确证法检测产超广谱β-内酰胺酶(ESBLs)菌.结果 130株KP主分布于呼吸内科,并以第二季度最多;对氨苄西林的耐药率高达95.31%,对第三代头孢菌素类抗生素的耐药率为41.27%～52.46%;最敏感抗生素为亚胺培南.产ESBLs肺炎克雷伯菌检出率为29.23%.结论肺炎克雷伯菌对抗生素的耐药性日趋严重,临床实验室应常规检测其是否产ESBLs.

Objective： To investigate the distribution and drug resistance of clinical isolates of Klebsiella pneumoniae in our hospital. Methods：Drug resistance were analyzed by K-B paper scraps spread method. The ESBL producing strains were detected by double disc test and confirmed test. Results：Most of the 130 strains of Klebsiella pneumoniae were distributed in the Division of Pulmonary Medicine,and were prevalent in the second quarter of the year. The resistant rates of the these isolates were 95.31% for Ampicillin,high up to 41.27%～52.46% for the third generation of Cephalosporins respectively. All of the strains were susceptible to imipenem. The frequency of ESBL-producing klebsiella, pneumoniae isolates were 29.23%. Conclusions：The resistance of the clinical isolates of Klebsiella pneumoniae is severe and the clinic laboratory should routinely detect ESBLs.