固定床生物反应器生产高滴度乙型脑炎病毒的研究

Study of the production process of Japanese encephalitisvirus by the fixed bed bioreactor

目的研究用固定床生物反应器生产乙型脑炎病毒的工艺。方法在生物反应器中培养V ero细胞,接种并培养乙型脑炎病毒。结果用工作体积3.5L的生物反应器生产出病毒滴度>8.0 lgLD50/m l的乙型脑炎病毒80L。结论用固定床生物反应器可以生产出高滴度的乙型脑炎病毒,为进一步生产高质量的乙型脑炎疫苗打下良好基础。

Objective：To study the production process of Japanese encephalitis virus using the fixed bed bioreactor. Methods ：Japanese encephalitis virus was inoculated and cultivated after the propagation of vero cells by bioreactor. Results ：80L Japanese encephalitis virus which titer is above 8. 01g LD50/ml was produced using 3. 5L working-volume bioreactor. Conclusion ：Japanese encephalitis virus with higher titer can be produced using the fix bed bioreactor.