腹泻患儿轮状病毒VP7基因分型与临床的关系

Relationship between VP7 Gene Types of Human Rotavirus and Clinical Characterist ic of Children with diarrhea

目的研究昆明地区2003年9～12月婴幼儿轮状病毒腹泻分子流行病学及临床特点.方法采用逆转录-聚合酶链法(RT-PCR)扩增婴幼儿腹泻大便样本中编码轮状病毒VP7蛋白的全基因片断,用巢式-聚合酶链反应(nested PCR)对扩增得到的VP7基因进行分型.结果 1.昆明地区2003年秋冬季50份标本能定型者32份(占64%).其中G3型占93.75%(30/32);混合型占6.25%(2/32),均为G1+G3型.本次分型中未见G2及G4型.2.2003年9～12月轮状病毒腹泻临床特点为病程短,脱水程度轻,腹泻次数少,电解质紊乱及酸中毒少见.结论1.2003年9～12月昆明地区轮状病毒流行株以G3型为主,与以往比较出现了流行株的转变.2.G1型感染的临床表现较G3型重.

Objective To ,study VP7 types of rotavirus and clinical characteristic of children with diarrhea in Kunming from 9 to 12 months in 2003. Methods Double - strand RNA of rotavirus extracted from stool samples was used as the template for reverse transcription of gene VP7, which was followed by nested polymerase chain reaction（PCR） for VP7 typing. Results 1. Among the 50 fecal specimens from fediatrid children with acute gastroenteritis during the epidemic season of 2003, 32 specimens （64 % ） were identified as rotavirus positive by RT-PCR. Of these, serotype G3 was found to be largely predominant and accounted for 93.75 % （30/32）. Coinfection of G1 and G3 accounted for 6.25% （2/32 ）. It was not found G2 arid G4.2, The clinical characteristic of children with diarrhea in Kunming from 2003-2004 was：course of disease was short;dehydration was light ;stool frequency was few acidosis and electrolytes disorders were not commonly ,seen. Conclusions 1. Serotype G3 is the prevalent VP7 types in Kunming. from 2003. A shift from G1 to G3 is observed in last two successive epidemic scasous of rotavirus epidemics. 2. The clinical manifestation of G1 is more severe than that of G3.