内皮素在单侧输尿管梗阻大鼠术侧肾脏的表达情况

Expression of Endothelin in Kidneys of Rats with Unilateral Ureteral Obstruction

目的观察内皮素(ET)在单侧输尿管梗阻(UUO)大鼠术侧肾脏的表达情况。方法建立左侧输尿管梗阻模型,设假手术组为对照组。4周后分别应用逆转录-聚合酶链反应(RT-PCR)和免疫组织化学方法检测ET-1 mRNA水平及蛋白质表达,并采用放射免疫法测定血浆与术侧肾脏的残余尿液中ET-1浓度。结果两组ET-1 mRNA和蛋白水平差异显著。病变组大鼠血浆和尿液ET-1浓度急剧上升,病理上呈现明显肾小管间质纤维化。结论ET-1参与UUO发生和发展的病理过程。

Objective To deteerrmine the expression of endothelin in kidneys of rats with unilateral ureteral obstruction （UUO）. Methods Rats were evenly divided into two groups. Some were subjected to complete ligation of left ureter and the others with sham operation were used as control group, Four weeks later, we examined the mRNA expressions of ET-1 in kidneys of all these rats using reverse transcription-polymerase chain reaction （RT-PCR） method. The inmmnohistochemistry analysis was carried out to investigate the protein level of ET- 1. The urinary concentration of ET- 1 was determined by radioimmunoassay. Results In UUO group, the uri- nary concentration of ET-1 increased substantially;both gene expression and protein level of ET-1 were much higher than those in control group. At the same time, tubulointerstitial fibrosis was present in the kidneys of rats with UUO. Conclusion ET-1 is involved in the pathogenesis of the injury of UUO.