SD大鼠骨髓基质细胞与多孔羟基磷灰石陶瓷支架体外粘附的实验研究

The study of cell adhesion characteristic between porous Hydroxyapatite and bone marrow stromal cells (BMSCs) in vitro

目的研究诱导培养的SD大鼠骨髓基质细胞(bone marrow stromal cells,BMSCs)在多孔羟基磷灰石陶瓷支架材料上的粘附和生长。方法SD大鼠骨髓基质细胞(bone marrow stromal cells,BMSCs)经矿化诱导培养、扩增后与多孔羟基磷灰石陶瓷支架体外复合培养,扫描电镜观察BMSCs在支架表面的贴附形态;同时以不同浓度细胞悬液接种多孔羟基磷灰石陶瓷支架材料,检测适宜的接种浓度及单位体积支架材料最多可粘附BMSCs数量。结果BMSCs在支架表面贴附良好,当接种浓度为2×106/ml时,单位体积的多孔羟基磷灰石陶瓷支架材料最多可粘附BMSCs数量为3.1×107cell/cm3。结论SD大鼠BMSCs在体外经矿化诱导培养可表达成骨细胞表型,诱导培养后的细胞与新型多孔羟基磷灰石陶瓷支架材料上具有良好的亲和能力。

Objective To study the adhesion characteristic in vitro between porous Hydroxyapatite and bone marrow stromal cells （BMSCs） that have been induced and proliferated. Methods BMSCs obtained from SD rat bone marrow were in vitro induced and proliferated and seeded onto prepared porous Hydroxyapatite,Their adhesion situation were analyzed by scanning electron microscope. To investigate the initial optimal cell seeding density between porous Hydroxyapatite and BMSCs by MTr automated colorrnetric microassay method. Results the most appropriate seeding density between them was 2.0×10^6cell/ ml. The maximal number which BMSCs could adhere to porous Hydroxyapatite is 3.1×10^7cell/cm^3. Conclusions The BMSCs were well adhered to new pattern porous hiphasic calcium phosphate nanocomposite.