再生障碍性贫血及慢性粒细胞性白血病患者骨髓单个核细胞Notch1表达研究

Study of Notchl expression on the bone marrow mononuclear cells from aplastic anemia patients and chronic myelogenous leukemia

目的了解再生障碍性贫血(AA)患者及慢性粒细胞性白血病(CML)惠者骨髓单个核细胞(BMMNC)Notchl表达情况,探讨Notchl与骨髓增生能力的相关性。方法应用半定量RT- PCR检测15例AA患者、8名正常对照者和10例CML患者BMMNC的Notchl的表达,并比较了8 例AA惠者化疗前后BMMNC的Notchl的表达,及10例CML患者化疗前后BMMNC的Notchl的表达。结果AA患者BMMNC的Notchl表达低于正常对照组(P<0.05);CML患者BMMNC的Notchl表达高于正常对照组(P<0.05);从患者化疗后Notchl表达上调(P<0.05);CML患者化疗后Notchl表达下调(P<0.05)。结论Notchl在AA患者BMMNC和正常人BMMNC中表达有差异,可能是AA患者骨髓增生能力低下的原因之一;AA患者化疗后BMMNC的Notchl表达水平上调,提示皮质激素和睾丸酮联合应用提高AA患者造血能力可能与Notchl上调有关。CML患者BMMNC的Notchl表达高于正常人,化疗缓解后Notchl表达下调,提示Notchl过度表达可能与CML的发生相关。

Objective To study the expression of Notchl on bone marrow mononuclear cells （BMMNC） from aplastic anemia （AA） and chronic myelogenous leukemia（CML） patients and to seek their relationship. Methods Semi-quantitative reverse transcriptase-polymerase chain reaction （RT-PCR） was used to investigate the expression of Notchl on BMMNCs in 15 AA patients 8controls and 10 CML patients, and the changes of expression after chemotherapy. Results Expression of Notchl on BMMNCs from AA patients was lower than that of control （P〈0. 05）, and was higher on BMMNCs from CML patients than that of control （P〈0. 05）. Notchl on the BMMNCs from AA patients was expressed higher after chemotherapy （P〈0. 05） and CML patients was lower after chemotherapy（P〈0.05）. Conclusion The expression of Notchl of AA patients was improved after effective chemothera- py. which may be one the reason of recovery of hematopoietic function. The expression of Notchl on BMMNCs from CML patients were much higher than that of control, which decreased after chemotherapy. It may be one of the factors which account for occurrence of CML.