雌激素对大鼠中医体质影响的研究

Study of estrogen on traditional Chinese medicine constitution in rats

目的研究外源性雌激素对不同性别、年龄大鼠中医体质(肾阳虚、阴虚火旺、肝郁)的影响.方法选用CD(SD)大鼠.6月龄(成年)雌、雄大鼠各分为对照组、雌激素组、治疗组.32日龄(幼年)雌性大鼠分为对照组、雌激素组、治疗组.32日龄(幼年)雄性大鼠分为对照组、雌激素组.各雌激素组和治疗组均用己烯雌酚注射,同时各治疗组分别用金匮肾气丸,逍遥丸+夏枯草,知柏地黄丸+龙胆草治疗反证.成年大鼠实验33～34d,幼年大鼠实验24d.实验结束后同步系统检测其生物学特性.然后以统一的评价标准评价外源性雌激素对不同性别、年龄大鼠中医体质(肾阳虚、阴虚火旺、肝郁)的影响.结果各雌激素组体质主要表现为寒热错杂,虚实相兼.与雄性比较,雌性雌激素组体质的热、实方面更为明显.这与雌激素对雌性来说属热、实因素吻合.但两个雌性雌激素组体温均下降,应属'真寒假热'.雌性雌激素组肝郁体质明显.不同年龄、性别的雌激素组间寒、热、虚、实要素明显不同.结论外源性雌激素对不同性别、年龄大鼠的中医体质(肾阳虚、阴虚火旺、肝郁)有不同影响.

Objective It is to research the influence of exogenous estrogen on traditional Chinese medicine constitution （dcficiency of kidney-Yang, hyperactivity of fire due to yin deficiency, stagnation of liver-Qi） in rats of different sex and age. Methods CD （SD） rats were chosen. Male and female rats of 6 months old （adult） were respectively divided into control group, cstrogen group and treatment group. Female rats of 32 days old （infant） were divided into control group, estrogen group and trcatment group. Male rats of 32 days old （infant） were divided into control group and estrogen group. All estrogen groups and treatment groups were injected with diethylstilbestrol. At the same time treatment groups were treated with Jinkuishenqi pill, Xiaoyao pill and bugle, Zhibaidihuang pill and Radix Gentiana as counter-evidence. Adult rats were treated for 33～34 days and infant rats were treated for 24 days. At the end of the experiment, biological characteristics were examined synchronously and systemically. The effect of exogenous estrogen on traditional Chinese medicine constitution （deficiency of kidney-Yang, hyperactivity of fire due to yin deficiency, stagnation of liver-Qi） in rats of different sex and age was evaluated with unified evaluation criterion. Results The estrogen groups showed intermingling of cold and heat syndrome, deficiency and sthenia syndrome. Compared with male rats, estrogen groups of female rats had more obvious heat and sthenia syndromes. This was accords with the fact that estrogen was a heat and sthenia factor for females. But the temperature in both estrogen groups of female rats decreased, which belonged to“true cold while false heat”. Female estrogen groups were distinct in stagnation of liver-Qi. Estrogen groups of different age and especially different sex were obviously different in cold, heat, deficiency and sthenia syndrome. Conclusion Exogenous estrogen has different effect on traditional Chinese medicine oonstitution （deficiency of kidney-Yang, hyperactivity of fire due to yin defidency, stagnation of liver-Qi） in rats of different sex and age.