紫外分光光度法测定盐酸丁卡因含量

Determination of the content of Tetracaine Hydrochloride by UV- Spectrohotometry

目的建立紫外分光光度法测定复方盐酸丁卡因含漱液中的盐酸丁卡因含量,有效控制药品质量。方法利用盐酸丁卡因最大吸收波长,在311nm处紫外分光光度法测定含量。结果盐酸丁卡因含量测定方法的线性范围为1～9μg·mL-1,其回归方程为y=0.076x+0.0045(n=5,r=1.0000),平均回收率为101.3%,RSD为0.78%(n=9)。在测定波长处空白溶液对盐酸丁卡因测定无干扰,样品溶液在7h内稳定。结论本法快速,简便,重现性好,可用于复方盐酸丁卡因含漱液的质量控制。

Objective To Determination of the content of Tetracaine Hydrochloride in Gargarisma Tetracaini Hydrochloride Co. by UV. Methods The Tetracaine Hydrochloride was determined at 311nm by ultraviolet spectrophotometry. Results the Tetracaine Hydrochloride had a good linearily in the concentration range of 1～9μg·mL^-1 （r=1.0000）. The average recovery was 101.3% with RSD of 0.78%（n=9） respectively.The sample solution was steady within 7 h. Conclustion This method is simple, rapid, accurate and can be used for the quality control of Gargarisma Tetracaini Hydrochloride Co..