粉防己碱对瘢痕成纤维细胞超微结构的影响

Effect of tetrandrine on the ultrastructure of scar fibroblasts

目的了解粉防己碱对瘢痕成纤维细胞的超微结构的影响。方法培养来自人体瘢痕的成纤维细胞,在加入粉防己碱后,用透射电镜观察其超微结构。结果使用粉防己碱后,成纤维细胞胞体变小,胞质突起变少,核变小,核仁不明显,细胞器变少且小。结论粉防己碱可影响瘢痕成纤维细胞的超微结构,从而可能降低其胶原蛋白等细胞外基质的合成。

Objective To investigate the effect of tetrandrine on the ultrastructure of scar fibroblasts. Methods The cells were cultured in DMEM containing 80ug/ml tetrandrine for 24h and the uhrastructure of the cells was observed under TEM. Results The fibroblasts became smaller with smaller nuclei and unclear nucleoli, and the organellae also became smaller and much less after tetrandrine treatment. Conclusion Tetrandrine has the effect on the uhrastructure, so it may inhibit cellular proliferation and collagen synthesis.