中期因子蛋白在非小细胞肺癌中的表达及其临床意义

Expression of midkine protein in non-small-cell lung cancer and its clinical significance

目的:探讨中期因子(MK)蛋白在非小细胞肺癌(NSCLC)中的表达水平、特点及其与NSCLC血管生成和预后的关系。方法:采用免疫组织化学方法检测44例NSCLC组织中MK蛋白表达和微血管密度(MVD),并与临床病理及预后指标作对照分析。结果:在癌旁及周围正常组织中,MK无表达;而在NSCLC癌细胞胞质中存在着MK的高表达,其表达阳性率为59.1%。MK表达与微血管密度增高、淋巴结转移显著相关(P<0.01),术后生存时间较MK阴性患者显著缩短(P<0.05)。MVD水平升高与淋巴结转移显著相关(P<0.01),且患者生存期显著缩短(P<0.05)。结论:MK蛋白在NSCLC中过表达,与肿瘤侵袭及血管生成有关,其表达水平能反映NSCLC的恶性程度,有可能作为NSCLC转移和预后分析的指标。

Purpose： To investigate the features of midkine（MK）protein expression and the correlation between MK expression with angiogenesis and prognosis in non-small-cell lung cancer（NSCLC）.Methods：Immunohistochemistry analysis for MK and microvessel density（MVD） were performed on 44 samples of tumor tissue.Results：No positivity expression was detected in either the lung tissue close to tumor or normal lung tissue.but 26 of 44 NSCLC tissue showed a positive reaction to MK immunostain.Significant correlation between MK expression with MVD enhancement and lymph metastasis in NSCLC was found.and the survival terms in NSCLC with MK expression were significantly shorter than those in NSCLC without MK expression.The enhanced MVD was related to lymph metastasis significantly,and the survival terms in NSCLC with enhanced MVD were significantly shorter than those in NSCLC without enham cement.Conclusions：In human non-small-cell lung cancer,MK overexpression at the protein level may very well be closely related to angiogenesis and metastasis.MK protein may be one of the predictors for prognosis in NSCLC.