四种钙拮抗剂对3T6成纤维细胞的调控

The effects of calcium antagonists on 3T6 fibroblasts

采用流式细胞技术观察不同剂量的四种钙拮抗剂（Ｃａ－Ａ）汉防己甲素（Ｔｅｔ）、硝苯啶（Ｎｉｆ）、维拉帕咪（Ｖｅｒ）及脑益嗪（Ｃｉｎ）对３Ｔ６成纤维细胞生长增殖的影响。结果表明它们不仅可阻断Ｇｌ→Ｓ期的进程，而且抑制Ｓ期ＤＮＡ的含量，但却增加Ｇｌ和Ｇ２期蛋白质的含量，使细胞呈现不平衡生长，最终死亡。其中Ｌｅｔ的作用最为显著，呈一定的量效关系，此作用与阻断钙离子内流无关。提示钙拮抗剂对３Ｔ６成纤维细胞生长增殖的抑制可能是其抗肝纤维化的机制之一。

By means of FCM the effects of calcium antagonists,tetrendrine(Tet),nifedipine (Nif), verapamil(Ver) and cinnacine(Cin),with different doses, on growth and proliferation of 3T6 fibroblasts were observed, The results showed that they could block the process of Gl→S phase,reduce DNA contents of S phase,as well as increase protein contents of G1 and G2 phases,the growth of cells lost its balance,then death occurred finally.Among these 4 calcium antagonists,Tet was one of the most prominant effect,and was related to the dosage.The conclusion is that the inhibition of growth and proliferation to 3T6 fibroblasts by calcium antagonists is probably one of the factors of the mechanism of their ability to anti-liver fibrosis.