八肽胆囊收缩素对大鼠胃平滑肌细胞收缩活动的作用

Contracting EHect of CCK-8 on Isolated Gastric Smooth Muscle Cells in Rats

通过游离平滑肌细胞的制备，观察ＣＣＫ－８对大鼠胃体，胃窦和幽门平滑肌细胞的直接作用并初步探讨其作用机制。结果表明：１．ＣＣＫ－８通过结合特异的受体而发挥对胃窦，幽门平滑肌细胞的收缩作用和对胃体平滑肌细胞的舒张作用。２．ＣＣＫ－８引起胃窦，幽门肌细胞收缩过程中，胞内Ｃａ￣（２＋）释放可能起重要作用；而ＣＣＫ－８引起胃体肌细胞舒张则可能与胞浆ｃＡＭＰ浓度的改变有关系。

he isolated gastric body.antrum and pylorus smooth muscle cells were used to investigate direct contractingeffect of CCK-8 on gastric smooth muscle cells.The results showed.(1)The contracting effect of CCK-8 on theantrum and pylorus smooth muscle cells and the relaxing effect on the gastric body muscle cells were exerted bybinding to specific CCK receptors.(2)During the contracting process.intracellular calcium may play a major rolewhile the cAMP concentration changes may be related to the relaxation of the body cells.