洋葱不同提取物抗真菌活性研究

Antifungal Action of Different Extracts from Bulbus Allii Cepae

[目的]观察采用不同提取工艺所得洋葱脂溶性部位对16株皮肤癣菌的体外抗真菌活性,为优化洋葱抗真菌活性产物的提取工艺提供依据。[方法]采用4种方法提取洋葱脂溶性部位,并用培养基药物浓度稀释法对产物进行抗真菌实验。[结果]乙醚提取的鲜洋葱脂溶性部位体外抗真菌活性较强,对上海絮状表皮癣菌等10株真菌的最低抑菌浓度(?)MIC≤6.25 mL·L-1,对其余菌株(?)MIC范围在12.5-50.ML·L-1,最低杀菌浓度((?)MFC)结果同抑菌实验,经过加热除水处理的乙醚提取样品几乎没有抗真菌活性。[结论]乙醚提取未经过加热处理的工艺较佳,所得到的鲜洋葱脂溶性部位具有较强的抑真菌活性。

[Objective] Antifungal action of fat-soluble extracts from Bulbus Allii Cepae （BAC） by different extraction technology on 16 strains of dennatophytes was observed to supply evidence for the optimization of extraction technology for BAC. [ Methods] Four methods were used for the extraction of fat-soluble extract from BAC and the antifungal action of the extract was tested by medium dilution method. [ Results] Fat-soluble extract freshly extracted from BAC by ether had a strong in-vitro antifungal action and its minimum inhibitory concentration （MIC） was not over 6.25 mL·L^-1 against 10 strains of fungi including Shanghai Epidermophytom floccosum and its MIC against other strains ranged 12.5-50.0 mL·L^-1. The minimum fungicidal concentration of fat-soluble extract freshly extracted from BAC by ether was as the same as MIC. Fat-soluble extract freshly extracted by ether had no antifungal action after water removal by heating. [ Conclusion] The extraction technology of freshly extracted by ether without heating is a better method for BAC and the fat-soluble extract extracted by this method has a better antifungal action.