中国白梨S基因研究Ⅰ 7个品种S基因型的确定和2个新S基因的鉴定

Studies of Chinese Pear S-Gene: Ⅰ Determination of 7 Cultivars of the S-genotype and Identification of Two New Allele of Self-incompatibility of Pyrus bretschneideri (Chinese pear)

梨S基因型的确定对授粉品种的选择具有指导作用.采用PCR-RFLP检测、DNA序列分析和田间杂交试验,鉴定了两个新S-RN ase基因,分别为S17-RN ase和S21-RN ase,同时确定了7个白梨品种的S基因型.S17-RN ase和S21-RN ase基因的内含子大小分别为142 bp和139 bp,HV区长度分别为16个和15个氨基酸,信号肽-P2的推导氨基酸长度分别为99个和98个氨基酸,信号肽大小均为27个氨基酸,C1大小均为11个氨基酸,C2大小均为12个氨基酸.白梨品种海棠酥、六瓣、恩梨、鸭梨、大核白、香椿、青梨等品种的S基因型分别为S1S12S19、S17S19、S1S19、S1S21、S16S19、S3S19、S19S19.该研究可为梨遗传改良和丰产栽培提供重要的理论依据.

To identify the S-genotype of the 7 Pyrus bretschneideri, PCR-RFLP system, DNA sequence and affinity analysis of cross experiment were performed. The S-genotype of the 7 cultivars such as Haitangsu, liuban, enli, boshanchi, dahebai, .riangchun and qingli are S1S12S19, S17S19, S1S19. S1S24, S16S19, S3S19 and S19S19 respectively. The findings of this research would provide an important scientific basis for the selection of pollination cultivars for the pears＇ cross experiment.