油茶优良无性系的RAPD分子鉴别

Identification of Oil Tea (Camellia oleifera) Superior Clones by RAPD Molecular Marker

油茶优良无性系是当前生产上的主要良种.通过采用RAPD分子标记方法,从180多个引物中筛选出22个具有多态性的随机引物进行扩增,得到141个位点,其中有91个是多态位点,以此为基础建立油茶优良无性系的RAPD分子鉴别体系.同时还探索出油茶叶片总DNA的分离技术和建立了RAPD反应体系,为油茶分子技术育种积累了相关的知识并奠定了DNA技术基础.

Superior clones of Oil Tea Camellia are the main elites in the actual production. For theis purpose. 22 primers out of 180 have been screened out and 141 RAPD markers can be amplified from these clones with 91 potymorphic loci. In addition, an efficient isolation of total DNA from Oil-Tea leaves and an applied RAPD reaction system have been established for Camellia oleifera. Thus, a sound ground has been laid down for the molecular breeding of Oil-Tea.