用秋水仙碱诱导非洲菊多倍体的研究

Study of Gerbera jamesonii Inducing Polyploid Plants with Colchicine

用秋水仙碱处理离体的非洲菊愈伤组织与丛生芽,研究了4种不同浓度的秋水仙碱对非洲菊芽增殖和染色体加倍的影响.结果表明:以0.05%的秋水仙碱溶液处理12 h效果较好,变异率达38.46%;叶片表皮细胞的气孔数量减少、体积增大以及植株叶大根粗等外部形态特征均可作为鉴别非洲菊多倍体的间接证据.

By treating buds and callus with cotchicines, the influence of Gerbera jamesonii on bud reproduction and chromosome doubling was studied. The re,cults show： it is the best for inducing mutation to treat buds and callus in cotchicine of 0, 05- for 12 h. The inducing rate is 38. 46%. This can be proved by the following evidence. The air holes of cells on the surface of the leaf of polyptoid plants are obviously decreasesed while their leaves are bigger and roots thicker than erver before.