复合诱变选育恶臭假单胞菌高产菌株的研究

Study of the Selected High-yielding Pseudomonas putida by Complication Mutation

以恶臭假单胞菌P seudom onas putid a野生株P s—FLAG 01为出发菌株,经超声波(U S)—硫酸二乙酯(DES)复合诱变后,筛选出一高产菌株P s—FLAG 05,其总酶活力为0.325 3 u/mL,比出发菌株提高25.89%;对其发酵培养基配方进行了优化,最佳配方为:玉米浆1.0%,葡萄糖3.5%,氯化钠0.15%,诱导剂4.5%,与原配方相比,发酵水平提高了13.03%.

After Pseudomoslas putiada wild strain Ps-FLAG01 was mutagenized by US-DES complication mutation, a high-yielding mutant Ps-FLAG05 was obtained. The enzyme activity of Ps-FLAG05 is 0. 325 3 u/mL which is 25.89% more than the wild strain. The optimal fermentation medium for Ps-FLAG05 is, Corn-steep liquor 1.0%, Glucose 3.5%, Nacl 0. 15% and Inducer 4. 5%. Contrasted to the original medium, the ferment-level was increased by 13. 03%.