摘要
本文利用全细胞膜片钳制技术，观察了白花前胡有效成分Ｐｒａ－Ａ对豚鼠单一心室肌细胞钙电流的影响．结果表明：１，１０，１００μｍｏｌ·Ｌ－１Ｐｒａ－Ａ可使钙电流峰值变小，随浓度增加，作用加强；１０μｍｏｌ·Ｌ－１Ｐｒａ－Ａ对钙电流的抑制具使用依赖性和频率依赖性．结果提示Ｐｒａ－Ａ对钙电流的阻断作用可能是白花前胡抗心律失常作用的机理之一；Ｐｒａ－Ａ可能对快速型心律失常有防治作用．
?-Praeruptorin A (Pra-A) is an effective component of Peucedanum praeruptorum Dunn, a Chinese traditional medicine. In present study, the effects of Pra-A on calcium current (I Ca ) in single ventricular cells of guinea pig heart were examined by using whole-cell patch clamp technique. The results showed that Pra-A (1, 10 and 100 μmol·L -1 ) decreased I Ca in a concentration-dependent manner, and that Pra-A (10 μmol·L -1 ) inhibited I Ca in a use-dependent and a frequency-dependent manner. These results suggested that Pra-A had a calcium channel blocking effect and Pra-A might be promising in the treatment of tachyarrhythmia.
出处
《中国药理学与毒理学杂志》
CSCD
北大核心
1996年第1期17-20,共4页
Chinese Journal of Pharmacology and Toxicology
基金
the
Science
Foundation
of
Ministry
of
Public
Health
关键词
白花前胡素
中药药理
心肌细胞
钙电流
豚鼠
Peucedanum praeruptorum Dunn
(±)-preaeruptorin A
patch clamp technique
calcium channel
calcium channel blockers
frequency-dependency
