三七总皂甙预处理对肿瘤坏死因子表达的影响

The Effect of Preconditioning with Panax Notoginseng Saponins on the Expression of Tumor Necrosis Factor-α

目的:观察三七总皂甙预处理对心肌缺血再灌注大鼠血清肿瘤坏死因子-α的影响。方法:采用开胸冠状动脉结扎建立缺血再灌注模型,25只雄性SD大鼠随机分为假手术组(n=4)、缺血再灌注组(IR组,n=7)及缺血预处理组(IPC组,n=7)及三七总皂甙预处理组(PNS预处理组,n=7)。分别于再灌注后2h测定各组血清肌酸磷酸激酶-MB(CK-MB)含量以及肿瘤坏死因子-α(TNF-α)。结果:PNS预处理组血清CK-MB含量为5098.43±1268.18U/L,明显低于IR组(17925.71±2022.74U/L,P(0.05);但高于IPC组(2304.29±540.04U/L,P<0.05)。IPC组及PNS预处理组血清TNF-α含量分别为1.08±0.08ng/ml、1.11±0.18ng/nl,均明显低于IR组(1.51±0.24ng/ml,两组均P<0.05);两组间无显著差异(P>0.05)。结论:PNS预处理早期通过抑制TNF-α释放对缺血再灌注心肌产生早期保护作用,但其保护作用不及缺血预处理。

Objective： To observe the change of tumor necrosis factor-α by preconditioning with Panax notoginseng saponins（PNS） on myocardial ischemia- reperfusion（IR） injury in rats. Methods： The model was established by left anterior descending coronary artery ligation under thoracotomy, preconditioning immediately preceded 45 man after left coronary artery occlusion followed by 120 minute＇s reperfusion. Twenty-five male Sprague Dawley rats were divided into four groups： sham operation group（n = 4）, IR group（n = 7）, IPC group（n = 7） and preconditioning with PNS group（n = 7）. The levels of creatine phosphokinase-MB （CK-MB） and tumor necrosis factor-α（TNF-α） in serum were measured. Results： The levels of serum CK-MB in the IPC group and preconditioning with PNS group were significantly lower than that in IR group （2304.29±540.04 U/L and 5098. 43±1268. 18 U/L vs. 17925. 71±2022. 74 U/L,P〈0. 05, respectively）. The levels of serum TNF-α in the IPC group and preconditioning with PNS group were significantly lower than that in IR group （1.08±0. 08 ng/ml and 1.11±0. 18 ng/ml vs. 1.51±0. 24 ng/ml,P〈0. 05, respectively）. Conclusion：Preconditioning with Panax notoginseng saponins can attenuate myocardial ischemia-reperfusion injury through restraining the levels of serum TNF-α, but the protection effect is inferior to IPC.