铁路危险品货运站空气颗粒提取物对大鼠肺细胞的损伤及抗氧化剂的干预作用

Effect of Abstract of Particulates Sampled From the Air of a Railway Transport Station of Hazard Chemicals on Rat TypeⅡ Pneumocytes and the Antagonism of Antioxidants

目的了解铁路危险品货运站空气颗粒提取物对大鼠肺Ⅱ型细胞的损伤,并探讨多种抗氧化剂的干预作用。方法原代培养SD大鼠肺Ⅱ型细胞分别以N-乙酰半胱氨酸(NAC)、维生素C(VC)和维生素E(VE)等抗氧化剂预处理,加入某铁路危险品货运站空气颗粒提取物染毒。用四甲基偶氮唑盐比色法、溴乙锭荧光法检测大鼠肺Ⅱ型细胞的生长情况和DNA交联的形成。结果经1.02～8.13mg/ml提取物染毒,大鼠肺Ⅱ型细胞的吸光度(A)值明显下降,有剂量-反应关系(r=0.962,P<0.05),经过终浓度为10～80mmol/L的NAC处理后,各实验组细胞的A值升高(P<0.01),加入终浓度为10mmol/L的NAC、VC和VE,各实验组细胞的A值升高,(P<0.01)。经1.02～8.13mg/ml空气颗粒提取物染毒,大鼠肺Ⅱ型细胞DNA交联率上升,有剂量-反应关系(r=0.981,P<0.01),加入终浓度为10mmol/L的VC和VE,各实验组DNA交联率下降(P<0.05)。结论铁路危险品货运站空气颗粒提取物能导致大鼠肺Ⅱ型细胞损伤并具有致DNA交联作用,NAC、VC和VE等抗氧化剂能拮抗这些作用。

Objective To know the effect of abstract of the particulates sampled from the air of the railway transport station of hazard chemicals on rat type Ⅱ pneumocytes and the antagonism of antioxidants. Methods The rat type Ⅱ pneumocytes cultured by routine method were pre-treated with antioxidants N-acetylcysteine （NAC）, Vitamin C and Vitamin E, then were exposed to the abstract of particulates. The damages of rat type Ⅱ pneumocytes and DNA cross-links were detected with MTT assay and ethidium bromide fluorescence assay. Results The abstract of particulates caused obvious damages and DNA cross-links in the rat type Ⅱ pneumocytes and the significant dose-effect relationship was seen. NAC, Vitamin C and Vitamin E reduced significantly the damages and DNA cross-links. Conclusion Abstract of the particulates sampled from the air of the railway transport station of hazard chemicals may induce the damages and DNA cross-links in rat type Ⅱ pneumocytes and these adverse effects can be antagonized by antioxidants such as NAC, Vitamin C and Vitamin E.