Graves’患者TSH抗独特型抗体检测及其意义

Determination of Anti-TSH Anti-idiotypic Antibody in Patients with Graves' Disease

根据自身免疫性甲状腺疾病发病机制，新的独特型－抗独特型免疫网络学说，用兔抗人ＴＳＨＡｂ检测ＴＳＨＡｂ_２。以正常人为对照，其结合率ｘ＋２ｓ为正常上限，大于此值为阳性。１００例ＴＲＡｂ阳性组有５２％患者ＴＳＨＡｂ_２阳性，５０例ＴＲＡｂ阴性组有４％患者ＴＳＨＡｂ２_阳性，两组有明显差异（Ｐ＜０．０５）。ＴＲＡｂ和ＴＳＨＡｂ_２正相关（ｒ＝０．５８２，Ｐ＜０．０５）。同时用制备的ＴＳＨ抗体吸附ＴＲＡｂ阳性血清后再测ＴＲＡｂ吸附前后有明显差异（Ｐ＜０．０５）。ＴＳＨＡｂ_２的检出为探讨Ｇｒａｖｅｓ’发病机制提供了一条新思路。

According to idiotypic anti-idiotypic immune network theory the new hypothesis of mechanism of autoimmune thyroid disease (AITD) had been stated. We determined the TSH anti-idiotypic antibody (TSHAb_2) in 100 patients with Graves' disease by using ￣( 125)I-hTSHAb and ￣(125)I-13B4 (monoclonal antibody of TSH ). In TRAb positive group, there is 52% positive rate. In TRAb negative group there is 4% positive rate. The two groups have obvious difference (P<0.05).In TRAb positive group, there ispositive related significance between TSHAb_2 and TRAb (r=0.582, P< 0.05).The determination of TSHAb_2 provides a new way to research the mechanism of Graves' disease.