大肠埃希氏菌ESBLs检测及其耐药性分析

THE DETECTION OF ESBLs-PRODUCING E.COLI AND THE RESISTANCE ANALYSIS TO 16 BLs SPECIES ANTIBIOTICS

目的:了解本地区人肠埃希氏菌分离株中超广谱β-内酰胺酶的阳性率,并分析产酶株对β-内酰胺类抗生素的耐约特点,为院内感染的预防与控制、治疗和流行病研究提供依据。方法:采用双纸片协同法检测所有菌株的ESBLs,并用Kirby—Bauer法检测了413株产超广谱β-内酰胺酶(ESBLs)的人肠埃希氏凶对16种β-内酰胺类抗生素耐约性,并对其标本米源和病区分布进行了分析。结果:ESBLs总检山率为45.0%,其中ESBLs检山率较高的科室为脑外科(100.0%)、肝胆外科(67.8%)呼吸科(65.0%)。所有人肠埃希氏菌分离株均对亚胺培南羽I关洛培南敏感。产ESBLs株对其它14种β-内酰胺类抗生素的耐药率均显著高于非产酶株。结论深圳地区ESBLs检山率高,其中脑外科ESBLs检山率高达100.0%,产ESBLs株对抗生素的耐约率普遍高于非ESBLs株。

Objective： To find out the ESBLs positive rate of isolated strains flow E.coli in Shenzhen area and analyse the resistance feature of ESBLs-producing bacteria to BLs species antibiotic, and to afford the basis for prevention and control, caring and epidemiology study.Methods： Double disk diffusion test was adopted to test ESBLs all of the strain＇s . And King· Bauer＇ s was used to test the resistance of 413 strains E.coli producing ESBLs to 16 BLs species antibiotics.Then these samples source and wards distribution were analysed.Results： The detection rate of ESBLs-producing bacteria was 45% .The bacteria which produced ESBLs were highly isolated from brain surgery （100%）, liver gall surgery （67.8%） and respiration ward （65.0%） .All of the isolated Strains of E.coli were sensitive to lmipenem and Meropenem.The resistance rate of strains producing ESBLs were higher than that of the strains non-producing ESBLs to offer 14 BLs species antibiotic significantly.Conclusion： ESBLs positive rate is highly in Shenzhen area. It＇s 100% in brain surgery, the resistance rate of the strains producing ESBLs to antibiotic is higher than that of the strains of non - producing ESBLs commonly.