纤维素三苯基氨基甲酸酯手性柱的评价及应用

Evaluation and Application of Cellulose Triphenylcarbamate Chiral Column

以微晶纤维素和苯基异氰酸酯为原料合成了纤维素三苯基氨基甲酸酯,经红外光谱和元素分析知微晶纤维素已酯化完全.用不同的方法(沉积法和蒸发法)将其涂敷于氨基丙烷化硅胶上制得了纤维素三苯基氨基甲酸酯手性柱,对柱进行了评价,并在正己烷-异丙醇(体积比为90∶10)为流动相,流速为1.0 mL.min-1,柱温25℃下拆分了12种手性物质.

Cellulose triphenylcarbamate （CTPC） were synthesized by the reaction of micrystalline cellulose with phenyliscocyanate in dry pyridine at 110 ℃. Elemental analysis and IR spectra indicated that most of the hydroxy groups of the microcrystalline cellulose had been converted into ester groups . The chiral stationary phases （CSP）were prepared by coating cellulose triphenylcarbamate on the aminopyopyl silica. The evaluation of column was achieved and 12 chiral substances were separated using hexane/2-propanol （90：10 volume ratio） as mobile phase at a flow rate of 1 mL·min^- 1 （ at 25℃）. And the results of the separation of chiral compound showed that the chiral stationary had good steroelectivity.