不同修复阶段创面肉芽组织中肿瘤坏死因子含量的变化

Tumor necrosis factor alpha (TNFa) in developing granulation tissue

采用大鼠背部刀伤模型与酶联免疫检测技术,动态观察伤后3、5、7天创面肉芽组织中内源性肿瘤坏死因子a(TNFa)含量的变化,并探讨这种变化与组织修复的关系。结果表明,伤后3个时间点创面肉芽组织中都检测出一定量的TNFa,且TNFa含量越低、创面面积越小、肉芽中蛋白含量越高、修复效果越好。结果提示创伤修复需要一定量TNFa的刺激,创面低浓度的TNFa可能较高浓度的TNFa更利于创伤修复。

The amount of tumor necrosis factor alpha (TNFa) in developing granulation tissue and their relation with the wound healing were studied. The model of deep skin defect in 18 Wistar rats was used and the developing gran- ulation tissues were harvested at davs 3, 5 and 7 after operation. The amounts of TNFa were measured with TNFa-ELISA kit. The results showed that TNFa could be found in all gran- ulation tissues, but its concentrations varied from 75 to 300pg/mg protein. The lower the TNF concentration, the smaller the wound area and the higher the amount of protein in granulation tissues the better the wound heal- ing. The results indicated that TNF is neces- sary for wound healing and the lower concen- tration of TNF may be more beneficial to wound healing than higher one.