高效液相色谱法测定跌打止痛巴布膏中大黄酚的含量

Determination of Chrysophanol in Diedazhitong Cataplasm by HPLC

目的:建立以高效液相色谱法测定跌打止痛巴布膏中大黄酚含量的方法。方法:色谱柱为KromasasilC18,流动相为甲醇-0.1%磷酸水(85∶15),流速为1.0ml/min,检测波长为254nm,进样量为10μl。结果:大黄酚进样量在0.6μg～4μg范围内与峰面积值线性关系良好(r=0.9994);平均加样回收率为99.23%(n=5,RSD=2.45%)。结论:本方法准确、可靠、重现性好。

OBJECTIVE：To determine the content of chrysophanol in Diedazhitong cataplsam by HPLC.METHODS：The samples were separated on a Kromasasil C18,methol - 0.1% phosphoric acid（85 ： 15） was taken as the mobile phase with a flow rate at 1.0ml/ min and detection wavelength at 254nm, the sample size was 10μl.RESULTS： Good linear relationship was achieved when the concentration range of chrysophanol was 0.6μg～4μg（ r = 0.9 994） ; The average recovery of chrysophanol was 99.23 % （ n = 5, RSD= 2.45 % ）. CONCLUSION： This method is accurate, reliable, and reproducible.