期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

RNA干扰技术在胃癌中的应用

下载PDF
导出
摘要 RNA干扰(RNAi)是近几年发展起来的一种阻抑基因表达的新方法,它是一个自然存在的以双链RNA为中介降解具有相同序列RNA的机制。目前,随着其分子机制研究的不断深入,RNAi技术已广泛应用于许多研究领域,尤其是在肿瘤方面,取得了一些突破性进展,为今后肿瘤基因治疗开辟了一条新的途径。
作者 郝建宏 顾琴龙
机构地区 上海第二医科大学附属瑞金医院普外科
出处 《国外医学（遗传学分册）》 2005年第4期204-206,共3页 Foreign Medical Sciences(Section of Genetics )
关键词 RNA 双链 基因表达调控 肿瘤 基因治疗 RNA干扰技术 肿瘤基因治疗 胃癌 RNAI技术 双链RNA
分类号 R735.2 [医药卫生—肿瘤]
  • 相关文献

参考文献14

  • 1Fire A, Xu S, Montgomery MK, et al. Potent and specific genetic interference by duble-stranded RNA in Caenorhabditis elegans. Nature,1998,391 (6669): 806-811.
  • 2Zamore PD, Tuschl T, Sharp PA, et al. RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals. Cell, 2000,101 (1): 25-33.
  • 3Hutvagner G, Zamore PD. RNAi: nature abhors a double-strand. Curr Opin Genet Dev, 2002,12(2) :225-232.
  • 4Elbashir SM, Lendeckel W, Tushl T. RNA interference is mediated by 21- and 22-nucleotide RNAs. Genes Dev, 2001,15(2): 188-200.
  • 5Sijen T, Flesnor J, Simmer F, et al. On the role of RNA amplification in dsRNA-triggered gene silencing. Cell, 2001,107(4) :465-476.
  • 6Lipardi C, Wei Q, Paterson BM. RNAi as random degradative PCR:siRNA primers convert mRNA into dsRNAs that are degraded to generate new siRNAs. Cell, 2001,107(3) :297-307.
  • 7Elbashir SM, Harborth J, Lendeckel W, et al. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature,2001,411 (6836) :494-498.
  • 8Jinawath N, Furukawa Y, Nakamura Y. Identifcation of NOL8, a nucleolar protein containing an RNA recognition motif (RRM), which was overexpressed in diffuse-type gastric cancer. Cancer Sci. 2004;95(5):430-435.
  • 9Xue Y, Bi F, Zhang X, et al. Inhibition of endothelial cell proliferation by targeting Racl GTPase with small interference RNA in tumor cells.Biochem Biophys Res Commun, 2004,320(4):1309-1315.
  • 10Hong L, Zhang Y, Han S, et al. Preparation and characterization of a novel monoclonal antibody specific to human ZNRD1 protein. Hybrid Hybridomics, 2004,23(1) :65-68.

二级参考文献15

  • 1Sahai E, Marshall CJ. Rho-GTPases and cancer [ J]. Nature Rev,2002, 2:133-142.
  • 2Clark EA, Golub TR, Lander ES, et al. Genomic analysis of metastasis reveals an essential role for RhoC [ J ]. Nature, 2000, 406(6795): 532 - 535.
  • 3Shi Y. Mammalian RNAi for the masses [J]. Trends Genet, 2002,19: 9-12.
  • 4Elbashir SM, Harborth J, Weber K, et al. Analysis of gene functionin somatic mammalian cells using small interfering RNAs [ J]. Methods, 2002, 26:199-213.
  • 5Yu JY, DeRuiter SL, Turner DL. RNA interference by expression ofshort-interfering RNAs and hairpin RNAs in mammalian cells [ J ].Proc Natl Acad Sci USA, 2002, 99: 6047 - 6052.
  • 6Kleer CG, van Golen KL, Zhang Y, et al. Characterization of RhoCexpression in benign and malignant breast disease: a potential newmarker for small breast carcinomas with metastatic ability [ J ]. Am JPathol, 2002, 160(2): 579 -584.
  • 7Horiuchi A, lmai T, Wang C, et al. Up-regulation of small GTPases, RhoA and RhoC, is associated with tumor progression in ovariancarcinoma [J]. Lab Invest, 2003, 83(6): 861 -870.
  • 8Marionnet C, Lalou C, Mollier K, et al. Differential molecular profiling between skin carcinomas reveals four newly reported genes potentially implicated in squamous cell carcinoma development [ J]. Oncogene, 2003, 22(22): 3500 -3505.
  • 9Zamore PD. Ancient pathways programmed by small RNAs [J]. Science, 2002, 296(17): 1265 - 1269.
  • 10Elbashir SM, Harborth J, Lendeckel W, et al. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells[J]. Nature, 2001,411:494-498.

共引文献5

国外医学（遗传学分册）
2005年 第4期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部