摘要
目的探讨神经降压素(neurotensin,NT)在大鼠脑内对应激性胃溃疡的影响及机制.方法选择健康雄性Wistar大鼠122只,用脑室微量注射NT等和放射免疫检测方法,观察中枢内、外源性NT对大鼠束缚加水浸诱导的应激性胃溃疡的影响.结果在大鼠应激性胃溃疡产生的同时,其血浆内神经降压素样免疫活性物(NT-IR)的含量明显减少[(162.86±34.25)pg/mg vs(221.54±31.82)pg/mg,P＜0.05],而下丘脑、中脑、脑桥、延脑和垂体中NT-IR含量则明显升高[下丘脑:(127.66±9.61)pg/mg vs(74.34±19.63)pg/mg;中脑:(52.59±3.25)pg/mg vs(8.74±2.25)pg/mg;脑桥:(26.99±2.30)pg/mgvs(7.80±2.24)pg/mg;延脑:(49.56±4.17)pg/mg vs(17.26±6.55)pg/mg;垂体:(18.97±3.24)pg/mg vs(5.42±1.25)pg/mg;P＜0.01)];侧脑室注射抗NT血清后,大鼠应激性胃溃疡的产生明显加重(溃疡指数:102.50±11.45 vs 91.38±9.12,P＜0.05);侧脑室微量注射NT,大鼠应激性胃溃疡的产生明显减轻,且呈明显的量效依赖关系(溃疡指数:5μg NT:82.25±7.69;10μg NT:76.99±7.71;20μg NT:50.38±13.13;30μg NT:11.25±4.65 vs 86.00±6.48,P＜0.05～0.01);经皮下注射消炎痛后,侧脑室注射NT,NT的胃粘膜细胞保护作用消失(溃疡指数:86.00±11.61 vs 88.80±12.88,P＞0.05).结论大鼠中枢内、外源性NT对胃粘膜细胞具有保护作用,该保护作用可能与前列腺素的合成有关.
Objective To investigate the cytoprotective effect and mechanism of NT on stress-induced gastric ulcers. Methods 122 Wistar rats were microinjected with NT, anti-NT serum or indomethacin into lateral ventricle. The NT concentration in plasma were detected by radio-immunoassay （RIA）. Results The NT in plasma were significantly decreased during stress ulcer induction, but increased in the hypothalamus, brain stem and pituitary. The stress induced ulcers was markedly promoted by microinjecting of anti-NT serum into the ventricle; and relieved by injecting of NT into the ventricle is a dose-dependent manner. After a subcutaneous injection of indomethacin, the cytoprotective effect of NT disappeared. Conclusion Endogenous and exogenous NT in the brain of rats had cytoprotective effect. The effect is probably due to the synthesis of prostaglandin.
出处
《中国行为医学科学》
CSCD
2005年第9期775-776,共2页
Chinese Journal of Behavioral Medical Science
基金
青岛大学医学院留学回国博士科研启动资金资助(600-411)
