铜对体外仔猪软骨细胞增殖和细胞骨架的影响

Effect of copper on proliferation and cellular skeleton in the young pig chondrocytes in vitro

体外分离、培养仔猪关节软骨细胞,在细胞培养液中分别添加铜0、7.8、15.6、31.2、62.5μmol/L。结果表明,软骨细胞在4种铜浓度中可存活并增殖,但随铜浓度的增加,其存活率、增殖率、3H-TdR掺入率有明显的差异,且能破坏软骨细胞骨架。培养液中添加铜31.2μmol/L,对软骨细胞的增殖作用最强,增殖率、3H-TdR掺入数显著高于对照组(P<0.01),软骨细胞形态及骨架均正常。表明31.2μmol/L铜浓度是促进体外软骨细胞增殖的最适浓度。

Chondrocytes from the lateral troehler ridge of the distal femur in one-day-old pig were cultured in medium supplemented with 0, 7.8, 15.6, 31.2, 62.5 μmol/L copper. DNA synthesis in chondrocytes was measured by ^3H-thymindine incorporation. The results showed that proliferation-promoting activity and incorporation of ^3H-thymindine in chondrocytes increased in culture medium added with 7.8, 15.6, 31.2 and 62.5 μmol/L Cu. The optimal copper concentration for the proliferation-promoting activity of chondrocytes was 31.2 μmol/L.