静电场对红细胞悬液冻结特性的影响

THE EFFECT OF EXTERNAL ELECTRIC FIELD ON THE FREEZING CHARACATERISTICS OF RED BLOOD CELLS SUSPENSION

本文以红细胞悬液为研究模型,通过在对其进行慢速冻结的过程中引入静电场,具体研究了静电场对降温过程中红细胞悬液冻结特性的影响。实验结果表明:静电场在一定程度上改变了冰晶的形成与生长特性,抑制了晶核的形成, 使晶枝出现不对称生长,并减缓了冰晶的生长速度,这些影响随着场强的增加而逐渐加强。在较强的静电场的影响下,冰晶明显变粗,最终成为块状,细胞也不再与冰晶分离而是完全融入粗大的冰晶之中,在冻结的末期,细胞不再受到冰晶挤压,从而减少了其所受的机械性损伤。

Using suspension of RBC as a “model solution system”, a high voltage DC electric field is introduced into its low-cooling-rate freezing process. How can an external static electric field affect the formation and growth characteristics of the ice during freezing is investigated？ It was found that the presence of external electric field has restrained the nucleation and developed process of ice crystals in the suspension during freezing. Under a strong electric field, the stems of ice crystals changed into board-like ones. The ceils were frozen within the ice crystal thoroughly; the mechanical damage caused by the interaction between the ice crystals and cells during freezing was alleviated remarkably.